Journal: JCI Insight
Article Title: C-terminal FGF-23 production coupling with aldosterone via FAM20C and predicting cardiovascular events in primary aldosteronism
doi: 10.1172/jci.insight.166461
Figure Lengend Snippet: ( A ) The dose-dependent effect of aldosterone on the upregulation of cFGF-23, n = 4/group. ( B ) The upregulation of cFGF-23 after aldosterone treatment remains unaffected by the furin inhibitor, n = 3/group. ( C ) Silencing Fam20C (shRNA) effectively restored the upregulated cleavage of cFGF-23, n = 3/group. Statistical analysis was conducted using 1-way ANOVA with Bonferroni’s post hoc correction for multiple comparisons (* P < 0.05, vs. control group). The graph data are presented as mean ± SEM.
Article Snippet: For Western blot analysis, polyclonal anti-furin antibody (sc-20801, obtained from Santa Cruz Biotechnology); mouse monoclonal antibodies targeting iFGF-23 and cFGF-23 (MAB26291, acquired from R&D Systems, Bio-Techne), with the distinction between the two made based on their molecular sizes on a gel; rabbit polyclonal FAM20C antibody (25395-1-AP, sourced from Proteintech); and rabbit polyclonal anti-GAPDH antibody (MAB374, obtained from MilliporeSigma) were utilized.
Techniques: shRNA, Control